Research teams

Cell Differentiation and Polarity

DIPOL 9 members

Gene editing in camelina
CRISPR-Cas9 editing of the 3 genes coding for delta-12-desaturase (FAD2) in Camelina sativa led to the accumulation of oleic acid in the oil with contents ranging from 10 to 62%. The different allelic combinations on the 3 homeologous genes allow an unbiased analysis of the quantitative traits associated with these genes and uniquely contribute to the creation of genetic variability for the improvement of this species (Morineau, et al. 2016).

Two edited lines of oleic camelina were evaluated in the field for the first time in Europe in 2018 and 2019 (Faure and Napier eLife 2018). Two gene edited camelina were evaluated in field trials 2018 and 2019 at Rothamsted Research Station (UK).


Nutritional impact of seed sphingolipids
Sphingolipids appear as a promising class of components susceptible to prevent the onset of the metabolic syndrome (MetS). Gut availability and effects of Camelina sativa sphingolipids were investigated in a mouse model of dietary-induced metabolic syndrome (Hermier et al. 2020). Seed meals from two Camelina sativa lines enriched respectively in C24- and C16- NH2-glycosyl-inositol-phosphoryl-ceramides (NH2GIPC) were used in hypercaloric diets.

After 5 weeks on these two hypercaloric diets, two markers of the MetS were alleviated (adiposity and insulin resistance) as well as inflammation markers and colon barrier dysfunction. A more pronounced effect was observed with the C16-NH2GIPC-enriched HC diet, in particular for colon barrier function. Despite a lower digestibility, C16-NH2GIPC were more prevalent in the intestine wall. Sphingolipids provided as camelina meal can therefore counteract some deleterious effects of a hypercaloric diet in mice at the intestinal and systemic levels. Interestingly, these beneficial effects seem partly dependent on sphingolipid acyl chain length. 
Cell Differentiation and Polarity

Leader:

Jean-Denis Faure
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