Seminar Prof Mathieu Ingouff
New live-imaging sensors to track CG methylation maintenance in living cells of Arabidopsis - Thursday, July 10th 2025 11:00 am, INRAE, Versailles
Cytosine methylation (mC) is an epigenetic mark that predominantly occurs at CG sequences in most eukaryotes and contributes to the regulation of gene and transposon activity. During DNA replication, the two symmetrically CG methylated parental strands separate and unmethylated DNA is synthesized, resulting in two hemimethylated DNA daughter molecules (one strand in methylated and the other is not). An evolutionarily conserved enzymatic machinery is specifically recruited to these transient hemimethylated regions to faithfully restore the parental CG methylation (mCG) pattern in the daughter cells. Recent data showed that mCG maintenance occurs further in the G2 phase in mammals.
By contrast, little is known about how and when CG methylation maintenance is achieved in plants. We have recently developed genetically encoded fluorescent sensors that allow us to visualise the entire process of CG methylation maintenance in living Arabidopsis cells. I will present the spatiotemporal dynamics of mCG maintenance during vegetative and reproductive phases. Notably, these demonstrate that mCG maintenance also occurs in a biphasic manner during the cell cycle.
Mathieu Ingouff, Epigenetic Regulations and Seed Development, Institut de Recherche pour le Développement (IRD), Université de Montpellier
Invitation: Filipe Borges, "Epigenetics, Reproduction and Transposable Elements" EPIREP team
In connection with the research developed at the Institute Jean-Pierre Bourgin for Plant Sciences.
By contrast, little is known about how and when CG methylation maintenance is achieved in plants. We have recently developed genetically encoded fluorescent sensors that allow us to visualise the entire process of CG methylation maintenance in living Arabidopsis cells. I will present the spatiotemporal dynamics of mCG maintenance during vegetative and reproductive phases. Notably, these demonstrate that mCG maintenance also occurs in a biphasic manner during the cell cycle.
Mathieu Ingouff, Epigenetic Regulations and Seed Development, Institut de Recherche pour le Développement (IRD), Université de Montpellier
Invitation: Filipe Borges, "Epigenetics, Reproduction and Transposable Elements" EPIREP team
In connection with the research developed at the Institute Jean-Pierre Bourgin for Plant Sciences.
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