David Rapoport wins second prize in poster contest at INRAE Microscopists Network days 2025
David Rapoport is a PhD student in the "Dynamics and Engineering of Lipidic Compartiments" DECLIC team, supervised by Marine Froissard, leader of the team, and Frédéric Jamme, Synchrotron SOLEIL. His poster awarded was one of the 23 posters presented during the 14th Scientific and Technical Days of the INRAE Microscopists’ Network RmuI. It concerns the "Deep-UV label free imaging for Lipid Droplets: overcoming the limit of fluorescence microscopy". This prize was awarded following a vote by the workshop participants.
PhD project - Integrative study of interactions between Lipid Droplets / Orthoflavivirus capsid proteins
Orthoflavivirus – such as Dengue, Zika, Yellow Fever, etc. – are emerging viruses transmitted by mosquitoes or ticks and are likely to spread due to global warming. Their coding RNA is encapsidated by capsid proteins (CPs) that have the unique ability to interact with Lipid Droplets (LDs). Viruses hijack LDs and use them as platforms for the assembly of new viral particles. This interaction between LDs and CPs represents a promising target for the development of innovative therapeutic strategies.
In cells, neutral lipids are stored in LDs, small organelles involved in many cellular pathways. LDs intern organization and interactions with proteins are poorly understood. We are investigating the nature of the interaction between CPs and LDs using and developing various techniques, such as confocal or label-free imaging, biochemistry analysis of lipids or structural studies of CPs – LDs complexes.
Poster abstract - Deep-UV label-free imaging of Lipid Droplets
Imaging occupies a central place in LDs study. Fluorescent tags allow direct visualisation in cells. These methods are harmful for LDs and proteins dynamics, chemical and physical characteristics.
At Synchrotron SOLEIL we develop a new imaging approach using Synchrotron Deep-UV illumination, enabling label-free imaging of LDs. This is made possible by the non-fluorescent and highly absorbent properties of lipids at 275nm and a low wavelength beam provided by Synchrotron installation. This low wavelength also provides better spatial resolution. Adapt this microscope – used to observe rocks from the Mars planet – on living organisms is a real challenge! Our method allows imaging of LDs in their most native environment revealing new clues about their internal structure and dynamics.
Research developed at the Institute Jean-Pierre Bourgin for Plant Sciences.
PhD project - Integrative study of interactions between Lipid Droplets / Orthoflavivirus capsid proteins
Orthoflavivirus – such as Dengue, Zika, Yellow Fever, etc. – are emerging viruses transmitted by mosquitoes or ticks and are likely to spread due to global warming. Their coding RNA is encapsidated by capsid proteins (CPs) that have the unique ability to interact with Lipid Droplets (LDs). Viruses hijack LDs and use them as platforms for the assembly of new viral particles. This interaction between LDs and CPs represents a promising target for the development of innovative therapeutic strategies.
In cells, neutral lipids are stored in LDs, small organelles involved in many cellular pathways. LDs intern organization and interactions with proteins are poorly understood. We are investigating the nature of the interaction between CPs and LDs using and developing various techniques, such as confocal or label-free imaging, biochemistry analysis of lipids or structural studies of CPs – LDs complexes.
Poster abstract - Deep-UV label-free imaging of Lipid Droplets
Imaging occupies a central place in LDs study. Fluorescent tags allow direct visualisation in cells. These methods are harmful for LDs and proteins dynamics, chemical and physical characteristics.
At Synchrotron SOLEIL we develop a new imaging approach using Synchrotron Deep-UV illumination, enabling label-free imaging of LDs. This is made possible by the non-fluorescent and highly absorbent properties of lipids at 275nm and a low wavelength beam provided by Synchrotron installation. This low wavelength also provides better spatial resolution. Adapt this microscope – used to observe rocks from the Mars planet – on living organisms is a real challenge! Our method allows imaging of LDs in their most native environment revealing new clues about their internal structure and dynamics.
Research developed at the Institute Jean-Pierre Bourgin for Plant Sciences.
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