Since my university studies, I have been interested in the secretory pathways and dynamics of intracellular compartments. After a PhD on regulated exocytosis in 2003 and a post-doctorate on the regulation of cell trafficking by ubiquitination, I joined INRAE in 2005 to work on lipid droplet.
Current projects aimed at
- Knowledge improvement of the structure of lipid droplet, lipids and proteins, studied in vitro and in cellulo by biophysical and imaging approaches (collaborations with IJPB DYSCOL, SOLEIL synchrotron ALBA)
- Comparative study of class I (integral) proteins from lipid droplet in the yeast model using a heterologous expression approach. We study the structural and functional convergence of plant, animal and viral proteins to better understand their role in the dynamics of oil accumulation.
- Exploring lipid diversity in yeast, with a particular interest in the production of omega-3 essential fatty acids in Debaryomyces species (collaborations with CIRM Levures, INRAE SayFood).
- Genetics of metabolic pathways and lipid body dynamics.
- Imaging with the use of specific markers (dyes or labelled proteins) or the development of non-labelled imaging by synchrotron radiation.
- Biochemistry with the development of specific fractionation and analysis methods for lipid-rich cellular compartments
- Biophysics using protocols and techniques compatible with the study of an oil-in-water emulsion.
- Improving the quality and extraction of edible vegetable oils
- Understanding the mechanisms of lipid accumulation in human diseases such as obesity, diabetes, hepatic cirrhosis, viral infections such as hepatitis C, zika or dengue fever.
- Identification of new sources of omega 3 or enzymes, for animal and human food, which are environmentally friendly.