Seminar Dr Andreas Niebel
The pioneer transcription factor NF-YA1 regulates nodule development via lncRNAs and transcription factors - Thursday, 9th April 2026 11 am, INRAE, Versailles
The transcription factor NF-YA1 regulates nodule development during the legume-Rhizobia symbiosis[1,2,3,4]. NF-Y transcription factors specifically bind CCAAT boxes in promoters have in addition been shown to act as pioneer transcription factors in both animals and plants[5,6]. Our working hypothesis is that NF-YA1 also acts as a pioneer transcription factor in Medicago truncatula regulating the root to nodule transition. In recent years, long non-coding RNAs (lncRNAs) have been described as a new class of regulators with roles in a wide range of cellular regulatory mechanisms, including the control of gene expression via chromatin modifications[7]. Comparative transcriptomics revealed numerous NF-YA1-dependent transcription factors (TF) and several lncRNAs activated during early steps of the legume-rhizobium symbiosis. In animals, NF-Y interacts with the lncRNA PANDA. Using an RNA-immunoprecipitation (RIP) strategy, we identified functional homologs of PANDA in both Arabidopsis and Medicago that physically interact with NF-YA1. Interestingly nodules overexpressing MtBAMBOOs, AtBAMBOO and HsPANDA lncRNAs show multilobed nodules, indicating a cross-kingdom activity of lncRNAs interacting with NF-YA1 on nodule meristem establishment. Hence, lncRNA-interacting with or regulated by NF-YA1 may help to regulate gene expression and consolidate the pioneering activity of these transcription factors in the context of nodule development. Functional and expression studies suggesting a role of these lncRNAs and TFs during the Medicago - Rhizobia symbiosis will be presented.
Reférences
1. Laloum T, De Mita S, Gamas P, Baudin M, Niebel A. 2013. CCAAT-box binding transcription factors in plants: Y so many?. Trends in Plant Science 18:157–16, doi
2. Baudin M, Laloum T, Lepage A, Ripodas C, Ariel F, Frances L, Crespi M, Gamas P, Blanco FA, Zanetti ME et al. 2015. A phylogenetically conserved group of Nuclear Factor-Y transcription factors interact to control nodulation in legumes. Plant Physiology 169: 2761–2773, doi
3. Shrestha, A.; Zhong, S. H.; Therrien, J.; Huebert, T. et al. Lotus japonicus Nuclear Factor YA1, a nodule emergence stage-specific regulator of auxin signalling. New Phytologist, 229, n. 3, p. 1535-1552, Feb 2021, doi
4. Xiao TT, Schilderink S, Moling S, Deinum EE, Kondorosi E, Franssen H, Kulikova O, Niebel A, Bisseling T. 2014. Fate map of Medicago truncatula root nodules. Development 141: 3517–3528, doi
5. Tao Z, Shen L, Gu X, Wang Y and He Y. 2017. Embryonic epigenetic reprogramming by a pioneer transcription factor in plants. Nature. 2017 551(7678):124-128, doi
6. Olfield AJ. et al. Nature Comm 2019. NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region, doi
7. Fonouni-Farde, C., Ariel,F., Crespi M. (2021) Plant long noncoding RNAs: New players in the field of post-transcriptional regulations. Non-coding RNA 7 (12), 1-17, doi
Andreas Niebel, "Endosymbiotic infection and nodule development" ENOD, "Laboratoire des inetractions plantes-microbes-environnement" LIPM, Toulouse
Invitation : Andana Barrios, "Epigenetics and small RNAs" epiARN team
Reférences
1. Laloum T, De Mita S, Gamas P, Baudin M, Niebel A. 2013. CCAAT-box binding transcription factors in plants: Y so many?. Trends in Plant Science 18:157–16, doi
2. Baudin M, Laloum T, Lepage A, Ripodas C, Ariel F, Frances L, Crespi M, Gamas P, Blanco FA, Zanetti ME et al. 2015. A phylogenetically conserved group of Nuclear Factor-Y transcription factors interact to control nodulation in legumes. Plant Physiology 169: 2761–2773, doi
3. Shrestha, A.; Zhong, S. H.; Therrien, J.; Huebert, T. et al. Lotus japonicus Nuclear Factor YA1, a nodule emergence stage-specific regulator of auxin signalling. New Phytologist, 229, n. 3, p. 1535-1552, Feb 2021, doi
4. Xiao TT, Schilderink S, Moling S, Deinum EE, Kondorosi E, Franssen H, Kulikova O, Niebel A, Bisseling T. 2014. Fate map of Medicago truncatula root nodules. Development 141: 3517–3528, doi
5. Tao Z, Shen L, Gu X, Wang Y and He Y. 2017. Embryonic epigenetic reprogramming by a pioneer transcription factor in plants. Nature. 2017 551(7678):124-128, doi
6. Olfield AJ. et al. Nature Comm 2019. NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region, doi
7. Fonouni-Farde, C., Ariel,F., Crespi M. (2021) Plant long noncoding RNAs: New players in the field of post-transcriptional regulations. Non-coding RNA 7 (12), 1-17, doi
Andreas Niebel, "Endosymbiotic infection and nodule development" ENOD, "Laboratoire des inetractions plantes-microbes-environnement" LIPM, Toulouse
Invitation : Andana Barrios, "Epigenetics and small RNAs" epiARN team
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