Seminar Dr Clémentine Vitte
Transposable elements contribution to maize tissue differentiation - Thursday, April 3rd 2025 11 am - INRAE, Versailles
Due to their repetitive nature and the fact that they harbor regulatory elements, transposable elements (TEs) were early envisioned as drivers of the evolution of gene regulatory networks through their ability to spread ‘pre-built’ regulatory elements in the genome. These first models have been recently refreshed by recent large-scale analyses of eukaryotic genetic regulatory landscapes, showing that TEs from various superfamilies can participate in the rewiring of different gene regulatory networks involved in key biological functions in various eukaryotic species. Nevertheless, the molecular mechanisms underlying the generation of TE-driven networks have been only characterized for a few cases and remains to be investigated at a larger scale.
In a recent study on maize (Fagny et al., 2021), we used a systems biology approach to investigate the enhancer-driven regulatory network of two tissues at different stages: leaves at seedling stage and ear-covering leaves at flowering (husk). We showed that modules specific to seedling leaves are characterized by the response to hormones and macromolecule biogenesis and assembly, while those specific of husks are characterized by cell wall modification and response to abiotic stresses. Analysis of enhancers sequence revealed that two different TE superfamilies have shaped part of the regulatory network in the two tissues, TIR transposon Mutator in seedling leaf, and MITE Pif/Harbinger in husk. Further investigation of Pif/Harbinger MITE elements revealed that a single family has contributed to the dissemination of regulatory elements throughout the maize genome, opening new avenues for deciphering the dynamics of cis-regulatory elements in maize.
Clémentine Vitte, GEvAD - Génomique Evolutive et Adaptation des plantes Domestiquées, GQE- Le Moulon, IDEEV, Gif-sur-Yvette
Invitation : Oumaya Bouchabké-Coussa, "Cell Biology and Plant Regeneration" BCR team
En relation avec une recherche développée à l’Institut Jean-Pierre Bourgin - Sciences du Végétal.
In a recent study on maize (Fagny et al., 2021), we used a systems biology approach to investigate the enhancer-driven regulatory network of two tissues at different stages: leaves at seedling stage and ear-covering leaves at flowering (husk). We showed that modules specific to seedling leaves are characterized by the response to hormones and macromolecule biogenesis and assembly, while those specific of husks are characterized by cell wall modification and response to abiotic stresses. Analysis of enhancers sequence revealed that two different TE superfamilies have shaped part of the regulatory network in the two tissues, TIR transposon Mutator in seedling leaf, and MITE Pif/Harbinger in husk. Further investigation of Pif/Harbinger MITE elements revealed that a single family has contributed to the dissemination of regulatory elements throughout the maize genome, opening new avenues for deciphering the dynamics of cis-regulatory elements in maize.
Clémentine Vitte, GEvAD - Génomique Evolutive et Adaptation des plantes Domestiquées, GQE- Le Moulon, IDEEV, Gif-sur-Yvette
Invitation : Oumaya Bouchabké-Coussa, "Cell Biology and Plant Regeneration" BCR team
En relation avec une recherche développée à l’Institut Jean-Pierre Bourgin - Sciences du Végétal.
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