Thesis defense: Nathan Ferchaud
Thursday, April 3rd 2025 2 pm - INRAE, Versailles
Bacterial methioninases: structural biology, interactions with the cofactor, and quest for a candidate better suited to anticancer therapy
Cancer cells are much more sensitive to methionine deprivation than normal ones. Methionine gamma-lyases (MGLs) efficiently reduce methionine concentration, and have been proposed for cancer therapy. Their development has been hampered by their insufficient plasma stability, and the risk of severe allergic reactions. We studied the MGL of the cheese ripening bacterium Brevibacterium aurantiacum, and the binding of its PLP (pyridoxal phosphate) cofactor, whose leakage is responsible for the loss of plasma activity. We looked for a more stable MGL among thermophilic organisms, and identified a promising candidate.
Director: Pierre Briozzo, équipe : "Dynamique et structure des corps lipidiques" DYSCOL, INRAE, IJPB, Versailles, AgroParisTech
Jury members
> Béatrice Golinelli (Rapportrice) - CNRS, Collège de France, Paris
> Julien Dairou (Rapporteur) - LCBPT, Université Paris Descartes, Paris
> Florence Bordes (Examinatrice) - INSA Toulouse, Université de Toulouse, Toulouse
> Nicolas Leulliot (Examinateur) - CitCom, Université Paris Cité, Paris
Research developed at the Institute Jean-Pierre Bourgin for Plant Sciences in collaboration.
Cancer cells are much more sensitive to methionine deprivation than normal ones. Methionine gamma-lyases (MGLs) efficiently reduce methionine concentration, and have been proposed for cancer therapy. Their development has been hampered by their insufficient plasma stability, and the risk of severe allergic reactions. We studied the MGL of the cheese ripening bacterium Brevibacterium aurantiacum, and the binding of its PLP (pyridoxal phosphate) cofactor, whose leakage is responsible for the loss of plasma activity. We looked for a more stable MGL among thermophilic organisms, and identified a promising candidate.
Director: Pierre Briozzo, équipe : "Dynamique et structure des corps lipidiques" DYSCOL, INRAE, IJPB, Versailles, AgroParisTech
Jury members
> Béatrice Golinelli (Rapportrice) - CNRS, Collège de France, Paris
> Julien Dairou (Rapporteur) - LCBPT, Université Paris Descartes, Paris
> Florence Bordes (Examinatrice) - INSA Toulouse, Université de Toulouse, Toulouse
> Nicolas Leulliot (Examinateur) - CitCom, Université Paris Cité, Paris
Research developed at the Institute Jean-Pierre Bourgin for Plant Sciences in collaboration.
Back
