List of Key Analytical Equipments in the PO-Biochemistry
> Monolith N115 Microscale Thermophoresis (Managers: Alexandre de Saint Germain/Céline Boulard). This device analyzes the mobility of macromolecules within a thermal gradient to assess a wide range of molecular interactions. It efficiently determines the affinity (Kd) between proteins and ligands with minimal sample consumption.
> Tycho (Manager: Alexandre de Saint Germain). The Tycho is used for protein quality control and protein-ligand interaction detection by measuring thermal denaturation through intrinsic protein fluorescence. It delivers rapid results with minimal sample requirements.
> TECAN Multimode Plate Reader (Manager: Alexandre de Saint Germain). Supports plate readings from 6 to 384 wells for applications including absorbance, fluorescence (TRF, FRET), nucleic acid quantification (PicoGreen), fluorescent protein detection (GFP, RFP, YFP), and luminescence. This reader includes an injector module, cooling system, and polarized fluorescence measurement capability.
> Typhoon FLA 9500 Biomolecular Imager (Manager: Céline Boulard). A versatile laser scanner for fluorescence and radioactive (P32) imaging on gels, membranes, Petri dishes, and multi-well plates. It provides precise quantification of proteins, nucleic acids, and radioactive signals.
> Äkta Purifier and Prime Chromatography Systems (Managers: Alexandre de Saint Germain/Céline Boulard). These systems are utilized for protein purification via affinity, ion-exchange, and size-exclusion chromatography and support protein-protein interaction studies through gel filtration.
> LAS 4000 Gel Imager (Managers: Hakim Mireau/Marianne Azzopardi). A camera system for gel or membrane imaging using chemiluminescence (immunoblots) and fluorescence. It also supports imaging of seedlings or bacteria expressing luminescent proteins (split-Luc, luciferase fusions).
>TL-100 Ultracentrifuge (Managers: Hakim Mireau/Anne Marmagne/Marianne Azzopardi). An ultracentrifuge designed for small volumes at very high speeds (up to 120,000 g), suitable for cellular membrane pelleting, density gradient complex separation, and PEG precipitation.
> VIAFLOW96 Pipetting Robot (Manager: Alexandre de Saint Germain). The INTEGRA VIAFLOW96 robot automates simultaneous pipetting of 96 samples, enhancing reproducibility and minimizing pipetting errors.
> Monolith N115 Microscale Thermophoresis (Managers: Alexandre de Saint Germain/Céline Boulard). This device analyzes the mobility of macromolecules within a thermal gradient to assess a wide range of molecular interactions. It efficiently determines the affinity (Kd) between proteins and ligands with minimal sample consumption.
> Tycho (Manager: Alexandre de Saint Germain). The Tycho is used for protein quality control and protein-ligand interaction detection by measuring thermal denaturation through intrinsic protein fluorescence. It delivers rapid results with minimal sample requirements.
> TECAN Multimode Plate Reader (Manager: Alexandre de Saint Germain). Supports plate readings from 6 to 384 wells for applications including absorbance, fluorescence (TRF, FRET), nucleic acid quantification (PicoGreen), fluorescent protein detection (GFP, RFP, YFP), and luminescence. This reader includes an injector module, cooling system, and polarized fluorescence measurement capability.
> Typhoon FLA 9500 Biomolecular Imager (Manager: Céline Boulard). A versatile laser scanner for fluorescence and radioactive (P32) imaging on gels, membranes, Petri dishes, and multi-well plates. It provides precise quantification of proteins, nucleic acids, and radioactive signals.
> Äkta Purifier and Prime Chromatography Systems (Managers: Alexandre de Saint Germain/Céline Boulard). These systems are utilized for protein purification via affinity, ion-exchange, and size-exclusion chromatography and support protein-protein interaction studies through gel filtration.
> LAS 4000 Gel Imager (Managers: Hakim Mireau/Marianne Azzopardi). A camera system for gel or membrane imaging using chemiluminescence (immunoblots) and fluorescence. It also supports imaging of seedlings or bacteria expressing luminescent proteins (split-Luc, luciferase fusions).
>TL-100 Ultracentrifuge (Managers: Hakim Mireau/Anne Marmagne/Marianne Azzopardi). An ultracentrifuge designed for small volumes at very high speeds (up to 120,000 g), suitable for cellular membrane pelleting, density gradient complex separation, and PEG precipitation.
> VIAFLOW96 Pipetting Robot (Manager: Alexandre de Saint Germain). The INTEGRA VIAFLOW96 robot automates simultaneous pipetting of 96 samples, enhancing reproducibility and minimizing pipetting errors.
Platform head:
Hakim Mireau