Plant lipid droplet biotechnology for the production of viral membrane proteins: application to the E and M proteins of SARS-CoV-2
As part of a research collaboration with Core Biogenesis, a start-up founded by a former post-doc from the lab, we have developed a set of molecular biotechnology tools and biological systems for the expression of hydrophobic recombinant proteins, particularly difficult to produce in heterologous systems. These proteins are of particular interest for the production of viral antigens with low variability, in a context of effective, rapid and low-cost therapeutic responses. Addressing these proteins in a lipid-rich environment like the lipid droplet, enables the purification of recombinant proteins by simple flotation. Lipid droplets from Camelina sativa seeds containing the hydrophobic E and M antigens of Sars-Cov-2 have been produced in our laboratory on a small scale, with a theoretical yield calculated at 80 to 540 mg/kg of seeds.
Gissot et al. 2024 Biotech. J; doi: 10.1002/biot.202300512
As part of a research collaboration with Core Biogenesis, a start-up founded by a former post-doc from the lab, we have developed a set of molecular biotechnology tools and biological systems for the expression of hydrophobic recombinant proteins, particularly difficult to produce in heterologous systems. These proteins are of particular interest for the production of viral antigens with low variability, in a context of effective, rapid and low-cost therapeutic responses. Addressing these proteins in a lipid-rich environment like the lipid droplet, enables the purification of recombinant proteins by simple flotation. Lipid droplets from Camelina sativa seeds containing the hydrophobic E and M antigens of Sars-Cov-2 have been produced in our laboratory on a small scale, with a theoretical yield calculated at 80 to 540 mg/kg of seeds.
Gissot et al. 2024 Biotech. J; doi: 10.1002/biot.202300512
Leader:
Marine Froissard