Applestorm: A PEPR B-Best Project Coordinated by IJPB
Imaging biomass multiscale architecture
Presentation of the PEPR B-Best
The national research program, PEPR, B-Best – short for Biomass, Biotechnologies, Technologies for Green Chemistry and Renewable Energies – is being developed from 2023 to 2029 as part of the French government’s France 2030 investment program. With a budget of €70 million, it is co-led by IFPEN and INRAE. The program's goal is to bring together the scientific community to lay the groundwork for technical, organizational, and social innovations. These innovations aim to both understand and activate the levers for efficient biomass transformation to produce bio-based products and sustainable fuels.
Applestorm project
Coordinator: Alexis Peaucelle, "Primary Cell Wall" PAR team
Collaboration IJPB: Kalina Haas et Heman Höfte, "Primary Cell Wall" PAR team
Other collaborations: Anne Imberty, CERMAV, Grenoble, Bénédicte Bakan, Marc Lahaye et Richard Sibout, BIA, Nantes
Abstract
This project will harness new developments in the imaging of the chemistry, orientation and mechanical properties of cell wall polymers for the multiscale characterization of plant biomass and its derived products. It will focus on apple, pear and tomato fruits for primary cell walls and cuticle, and maize stems for secondary cell walls. Biomass will be characterized, before and after extraction, with correlative microscopy combining RAMAN, multiphotonic second harmonic generation microscopy, atomic force microscopy, spectral fluorescence life time imaging (sFLIM) and Direct Stochastic Optical Reconstruction Microscopy (dSTORM) super-resolution imaging. To extend the 3D isotropic resolution of dSTORM to >15nm and the range of detectable polymer epitopes, new specific and high affinity fluorescent nanoprobes will be developed through synthetic biology. The multiscale structural parameters can be used for the analysis of the biomass variability and for modeling of biomass quality traits. The extended dSTORM toolbox will also be used to map epitopes on isolated polysaccharides. This may eventually lead to the commercialization of equipment for polysaccharide characterization and quality control. Finally, the in situ mobility of cell wall modifying enzymes (pectin-esterases and cellulases) in different types of biomass will be assessed using single particle tracking and correlated with variation in pertinent biomass quality traits
STORM super-resolution microscope, IJPB news 29/11/23
Research developed at the Institute Jean-Pierre Bourgin for Plant Sciences in collaboration.
The national research program, PEPR, B-Best – short for Biomass, Biotechnologies, Technologies for Green Chemistry and Renewable Energies – is being developed from 2023 to 2029 as part of the French government’s France 2030 investment program. With a budget of €70 million, it is co-led by IFPEN and INRAE. The program's goal is to bring together the scientific community to lay the groundwork for technical, organizational, and social innovations. These innovations aim to both understand and activate the levers for efficient biomass transformation to produce bio-based products and sustainable fuels.
Applestorm project
Coordinator: Alexis Peaucelle, "Primary Cell Wall" PAR team
Collaboration IJPB: Kalina Haas et Heman Höfte, "Primary Cell Wall" PAR team
Other collaborations: Anne Imberty, CERMAV, Grenoble, Bénédicte Bakan, Marc Lahaye et Richard Sibout, BIA, Nantes
Abstract
This project will harness new developments in the imaging of the chemistry, orientation and mechanical properties of cell wall polymers for the multiscale characterization of plant biomass and its derived products. It will focus on apple, pear and tomato fruits for primary cell walls and cuticle, and maize stems for secondary cell walls. Biomass will be characterized, before and after extraction, with correlative microscopy combining RAMAN, multiphotonic second harmonic generation microscopy, atomic force microscopy, spectral fluorescence life time imaging (sFLIM) and Direct Stochastic Optical Reconstruction Microscopy (dSTORM) super-resolution imaging. To extend the 3D isotropic resolution of dSTORM to >15nm and the range of detectable polymer epitopes, new specific and high affinity fluorescent nanoprobes will be developed through synthetic biology. The multiscale structural parameters can be used for the analysis of the biomass variability and for modeling of biomass quality traits. The extended dSTORM toolbox will also be used to map epitopes on isolated polysaccharides. This may eventually lead to the commercialization of equipment for polysaccharide characterization and quality control. Finally, the in situ mobility of cell wall modifying enzymes (pectin-esterases and cellulases) in different types of biomass will be assessed using single particle tracking and correlated with variation in pertinent biomass quality traits
STORM super-resolution microscope, IJPB news 29/11/23
Research developed at the Institute Jean-Pierre Bourgin for Plant Sciences in collaboration.
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