Dynamique et Structure des Corps Lipidiques

Gissot L, Fontaine F, Kelemen Z, Dao O, Bouchez I, Deruyffelaere C, Winkler M, Da Costa A, Pierre F, Meziadi C, Faure JD, Froissard M (2023). E and M SARS-CoV-2 membrane protein expression and enrichment with plant lipid droplets. Biotechnol J, e2300512. PubMed | DOI

Chen X, Ferchaud N, Briozzo P, Machover D, Simonson T (2023). PLP-Dependent Enzyme Methionine γ-Lyase: Insights into the Michaelis Complex from Molecular Dynamics and Free Energy Simulations. Biochemistry, doi: 10.1021/acs.biochem.3c00355. PubMed | DOI

Bouchnak I, Coulon D, Salis V, D'Andrea S, Bréhélin C (2023). Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes. Front Plant Sci, 14:1193905. PubMed | DOI

Bělíček J, Ľuptáková, Kopecný D, Frömmel J, Vigouroux A, Ćavar Zeljković S, Jagic F, Briozzo P, Kopečný DJ, Tarkowski P, Nisler J, De Diego N, Moréra S, Kopecná M (2023). Biochemical and structural basis of polyamine, lysine and ornithine acetylation catalyzed by spermine/spermidine N-acetyl transferase in moss and maize. Plant J, doi: 10.1111/tpj.16148. PubMed | DOI

de L TovillaCoutino M, Passot S, Trelea IC, Ropers MH, Gohon Y, Fonseca F (2023). Multi-objective optimization of frozen and freeze-dried Lactobacillus delbrueckii subsp. bulgaricus CFL1 production via the modification of fermentation conditions. J Appl Microbiol, lxad003. PubMed | DOI

Chardot T (2022). A comparison of natural and induced diversity in plant oils. OCL, 29:34. DOI

Chen X, Briozzo P, Machover D, Simonson T (2022). A Computational Model for the PLP-Dependent Enzyme Methionine ?-Lyase. Front Mol Biosci, 9:886358. PubMed | DOI

Mameri H, Gaudin JC, Lollier V, Tranquet O, Brossard C, Pietri M, Marion D, Codreanu-Morel F, Beaudouin E, Wien F, Gohon Y, Briozzo P, Denery-Papini S (2022). Critical structural elements for the antigenicity of wheat allergen LTP1 (Tri a 14) revealed by site-directed mutagenesis. Sci Rep, 12(1):12253. PubMed | DOI

Lopez C, Novales B, Rabesona H, Weber M, Chardot T, Anton M (2021). Deciphering the properties of hemp seed oil bodies for food applications: Lipid composition, microstructure, surface properties and physical stability. Food Res Int, 150(Pt A):110759. PubMed | DOI

Nisler J, Kopecný D, Pekná Z, Koncitíková R, Koprna R, Murvanidze N, Werbrouck SPO, Havlícek L, De Diego N, Kopecná M, Wimmer Z, Briozzo P, Moréra S, Zalabák D, Spíchal L, Strnad M (2021). Diphenylurea-derived cytokinin oxidase/dehydrogenase inhibitors for biotechnology and agriculture. J Exp Bot, 72(2):355-370. PubMed | DOI

Jamme F, Cinquin B, Gohon Y, Pereiro E, Refregiers M, Froissard M (2020). Synchrotron multimodal imaging in a whole cell reveals lipid droplet core organization. J Synchrotron Radiat, 27:772-778. PubMed | DOI

Masclaux-Daubresse C, D'Andrea S, Bouchez I, Cacas JL (2020). Reserve lipids and plant autophagy. J Exp Bot, 71:2854-2861. PubMed | DOI

Machover D, Rossi L, Hamelin J, Desterke C, Goldschmidt E, Chadefaux-Vekemans B, Bonnarme P, Briozzo P, Kopecný D, Pierige F, Magnani M, Mollicone R, Haghighi-Rad F, Gaston-Mathe Y, Dairou J, Boucheix C, Saffroy R (2019). Effects in Cancer Cells of the Recombinant l-Methionine Gamma-Lyase from Brevibacterium aurantiacum. Encapsulation in Human Erythrocytes for Sustained l-Methionine Elimination. J Pharmacol Exp Ther, 369:489-502. PubMed | DOI

Miller C, Wells R, Mckenzie N, Trick M, Ball J, Fatihi A, Dubreucq B, Chardot T, Lepiniec L, Bevan MW (2019). Variation in expression of the HECT E3 ligase UPL3 modulates LEC2 levels, seed size and crop yield in Brassica napus. Plant Cell, D1419tpc.00577.02018:. PubMed | DOI

Rethore E, D'Andrea S, Benamar A, Cukier C, Tolleter D, Limami AM, Avelange-Macherel MH, Macherel D (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J, 99:302-315. PubMed | DOI

Ayme L, Arragain S, Canonge M, Baud S, Touati N, Bimai O, Jagic F, Louis-Mondésir C, Briozzo P, Fontecave M, Chardot T (2018). Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis. Sci Rep, 8:17254. PubMed | DOI

Deruyffelaere C, Purkrtova Z, Bouchez I, Collet B, Cacas JL, Chardot T, Gallois JL, D'Andrea S (2018). PUX10 Is a CDC48A Adaptor Protein That Regulates the Extraction of Ubiquitinated Oleosins from Seed Lipid Droplets in Arabidopsis. Plant Cell, 30:2116-2136. PubMed | DOI

Rigouin C, Croux C, Borsenberger V, Ben Khaled M, Chardot T, Marty A, Bordes F (2018). Increasing medium chain fatty acids production in Yarrowia lipolytica by metabolic engineering. Microb Cell Fact, 17:142. PubMed | DOI

Sossna-Wunder G, Hengstenberg W, Briozzo P, Deutscher J (2018). Photoinactivation of the Staphylococcus aureus Lactose-Specific EIICB Phosphotransferase Component with p-azidophenyl-beta-D-Galactoside and Phosphorylation of the Covalently Bound Substrate. J Mol Microbiol Biotechnol, 28:147-158. PubMed | DOI

Baud A, Ayme L, Gonnet F, Salard I, Gohon Y, Jolivet P, Brodolin K, Da Silva P, Giuliani A, Sclavi B, Chardot T, Mercere P, Roblin P, Daniel R (2017). SOLEIL shining on the solution-state structure of biomacromolecules by synchrotron X-ray footprinting at the Metrology beamline. J Synchrotron Radiat, 24:576-585. PubMed | DOI

Jolivet P, Ayme L, Giuliani A, Wien F, Chardot T, Gohon Y (2017). Structural proteomics: Topology and relative accessibility of plant lipid droplet associated proteins. J Proteomics, 169:87-98. PubMed | DOI

Kopecná M, Vigouroux A, Vilim J, Koncitíková R, Briozzo P, Hajkova E, Jaskova L, Von Schwartzenberg K, Sebela M, Moréra S, Kopecný D (2017). The ALDH21 gene found in lower plants and some vascular plants codes for a NADP(+) -dependent succinic semialdehyde dehydrogenase. Plant J, 92:229-243. PubMed | DOI

D'Andrea S (2016). Lipid droplet mobilization: The different ways to loosen the purse strings. Biochimie, 120:17-27. PubMed | DOI

Groeme R, Airouche S, Kopecný D, Jaekel J, Savko M, Berjont N, Bussieres L, Le Mignon M, Jagic F, Zieglmayer P, Baron-Bodo V, Bordas-Le Floch V, Mascarell L, Briozzo P, Moingeon P (2016). Structural and Functional Characterization of the Major Allergen Amb a 11 from Short Ragweed Pollen. J Biol Chem, 291:13076-13087. PubMed | DOI

Haili N, Louap J, Canonge M, Jagic F, Louis-Mondésir C, Chardot T, Briozzo P (2016). Expression of Soluble Forms of Yeast Diacylglycerol Acyltransferase 2 That Integrate a Broad Range of Saturated Fatty Acids in Triacylglycerols. PLoS One, 11:e0165431. PubMed | DOI

Hluska T, Dobrev PI, Tarkowska D, Frebortova J, Zalabák D, Kopecný D, Plihal O, Kokas F, Briozzo P, Zatloukal M, Motyka V, Galuszka P (2016). Cytokinin metabolism in maize: Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity. Plant Sci, 247:127-137. PubMed | DOI

Kopecný D, Koncitíková R, Popelka H, Briozzo P, Vigouroux A, Kopecná M, Zalabák D, Sebela M, Skopalova J, Frebort I, Moréra S (2016). Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site. FEBS J, 283:361-377. PubMed | DOI

Nisler J, Kopecný D, Koncitíková R, Zatloukal M, Bazgier V, Berka K, Zalabák D, Briozzo P, Strnad M, Spíchal L (2016). Novel thidiazuron-derived inhibitors of cytokinin oxidase/dehydrogenase. Plant Mol Biol, 92:235-248. PubMed | DOI

Ayme L, Jolivet P, Nicaud JM, Chardot T (2015). Molecular Characterization of the Elaeis guineensis Medium-Chain Fatty Acid Diacylglycerol Acyltransferase DGAT1-1 by Heterologous Expression in Yarrowia lipolytica. PLoS One, 10:e0143113. PubMed | DOI

Bouchez I, Pouteaux M, Canonge M, Genet M, Chardot T, Guillot A, Froissard M (2015). Regulation of lipid droplet dynamics in Saccharomyces cerevisiae depends on the Rab7-like Ypt7p, HOPS complex and V1-ATPase. Biol Open, 4:764-775. PubMed | DOI

Boulard C, Bardet M, Chardot T, Dubreucq B, Gromova M, Guillermo A, Miquel M, Nesi N, Yen-Nicolay S, Jolivet P (2015). The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes. Planta, 242:53-68. PubMed | DOI

Deruyffelaere C, Bouchez I, Morin H, Guillot A, Miquel M, Froissard M, Chardot T, D'Andrea S (2015). Ubiquitin-Mediated Proteasomal Degradation of Oleosins is Involved in Oil Body Mobilization During Post-Germinative Seedling Growth in Arabidopsis. Plant Cell Physiol, 56:1374-1387. PubMed | DOI

Dulermo T, Coze F, Virolle MJ, Méchin V, Baumberger S, Froissard M (2015). Bioconversion of agricultural lignocellulosic residues into branched-chain fatty acids usingStreptomyces lividans. Ocl, 23:A202. PubMed | DOI

Froissard M, Canonge M, Pouteaux M, Cintrat B, Mohand-Oumoussa S, Guillouet SE, Chardot T, Jacques N, Casaregola S (2015). Lipids containing medium-chain fatty acids are specific to post-whole genome duplication Saccharomycotina yeasts. BMC Evol Biol, 15:97. PubMed | DOI

Mameri H, Brossard C, Gaudin JC, Gohon Y, Paty E, Beaudouin E, Moneret-Vautrin DA, Drouet M, Sole V, Wien F, Lupi R, Larre C, Snegaroff J, Denery-Papini S (2015). Structural Basis of IgE Binding to alpha- and gamma-Gliadins: Contribution of Disulfide Bonds and Repetitive and Nonrepetitive Domains. J Agric Food Chem, 63:6546-6554. PubMed | DOI

Purkrtova Z, Chardot T, Froissard M (2015). N-terminus of seed caleosins is essential for lipid droplet sorting but not for lipid accumulation. Arch Biochem Biophys, 579:47-54. PubMed | DOI

Ayme L, Baud S, Dubreucq B, Joffre F, Chardot T (2014). Function and localization of the Arabidopsis thaliana diacylglycerol acyltransferase DGAT2 expressed in yeast. PLoS One, 9:e92237. PubMed | DOI

Miquel M, Trigui G, D'Andrea S, Kelemen Z, Baud S, Berger A, Deruyffelaere C, Trubuil A, Lepiniec L, Dubreucq B (2014). Specialization of oleosins in oil body dynamics during seed development in Arabidopsis seeds. Plant Physiol, 164:1866-1878. PubMed | DOI

Vermachova M, Purkrtova Z, Santrucek J, Jolivet P, Chardot T, Kodicek M (2014). Combining chymotrypsin/trypsin digestion to identify hydrophobic proteins from oil bodies. Methods Mol Biol, 1072:185-198. PubMed | DOI

Haili N, Arnal N, Quadrado M, Amiar S, Tcherkez G, Dahan J, Briozzo P, Colas Des Francs-Small C, Vrielynck N, Mireau H (2013). The pentatricopeptide repeat MTSF1 protein stabilizes the nad4 mRNA in Arabidopsis mitochondria. Nucleic Acids Res, 41:6650-6663. PubMed | DOI

Jamme F, Vindigni JD, Méchin V, Cherifi T, Chardot T, Froissard M (2013). Single cell synchrotron FT-IR microspectroscopy reveals a link between neutral lipid and storage carbohydrate fluxes in S. cerevisiae. PLoS One, 8:e74421. PubMed | DOI

Jolivet P, Acevedo F, Boulard C, D'Andrea S, Faure JD, Kohli A, Nesi N, Valot B, Chardot T (2013). Crop seed oil bodies: from challenges in protein identification to an emerging picture of the oil body proteome. Proteomics, 13:1836-1849. PubMed | DOI

Jolivet P, Deruyffelaere C, Boulard C, Quinsac A, Savoire R, Nesi N, Chardot T (2013). Deciphering the structural organization of the oil bodies in the Brassica napus seed as a mean to improve the oil extraction yield. Industrial Crops and Products, 44:549-557. PubMed | DOI

Kubienova L, Kopecný D, Tylichova M, Briozzo P, Skopalova J, Sebela M, Navratil M, Tache R, Luhova L, Barroso JB, Petrivalsky M (2013). Structural and functional characterization of a plant S-nitrosoglutathione reductase from Solanum lycopersicum. Biochimie, 95:889-902. PubMed | DOI

Le Marechal P, Decottignies P, Marchand CH, Degrouard J, Jaillard D, Dulermo T, Froissard M, Smirnov A, Chapuis V, Virolle MJ (2013). Comparative proteomic analysis of Streptomyces lividans Wild-Type and ppk mutant strains reveals the importance of storage lipids for antibiotic biosynthesis. Appl Environ Microbiol, 79:5907-5917. PubMed | DOI

Snegaroff J, Bouchez I, Smaali Mel A, Pecquet C, Raison-Peyron N, Jolivet P, Lauriere M (2013). Barley gamma3-hordein: glycosylation at an atypical site, disulfide bridge analysis, and reactivity with IgE from patients allergic to wheat. Biochim Biophys Acta, 1834:395-403. PubMed | DOI

Vindigni JD, Wien F, Giuliani A, Erpapazoglou Z, Tache R, Jagic F, Chardot T, Gohon Y, Froissard M (2013). Fold of an oleosin targeted to cellular oil bodies. Biochim Biophys Acta, 1828:1881-1888. PubMed | DOI

Acevedo F, Rubilar M, Shene C, Navarrete P, Romero F, Rabert C, Jolivet P, Valot B, Chardot T (2012). Seed oil bodies from Gevuina avellana and Madia sativa. J Agric Food Chem, 60:6994-7004. PubMed | DOI

Beopoulos A, Haddouche R, Kabran P, Dulermo T, Chardot T, Nicaud JM (2012). Identification and characterization of DGA2, an acyltransferase of the DGAT1 acyl-CoA:diacylglycerol acyltransferase family in the oleaginous yeast Yarrowia lipolytica. New insights into the storage lipid metabolism of oleaginous yeasts. Appl Microbiol Biotechnol, 93:1523-1537. PubMed | DOI

Jasinski S, Lécureuil A, Miquel M, Loudet O, Raffaele S, Froissard M, Guerche P (2012). Natural variation in seed very long chain fatty acid content is controlled by a new isoform of KCS18 in Arabidopsis thaliana. PLoS One, 7:e49261. PubMed | DOI